Practical Course Prof. Dr. Bernhard Hube

Candida albicans infection of the human oral epithelium - Fully booked!

Date
June 17th and 18th 2009
from 09:00 - 18:00
HKI, Beutenbergstr. 11a, Dept. Microbial Pathogenicity Mechanisms, Laborhaus 3, seminar room 1st floor

Course instructors
Dr. Duncan Wilson, Francois Mayer


Description
Oral C. albicans infections are characterised by adhesion of the fungus to the epithelium, invasion into host cells and tissue destruction. Techniques for analysing these stages of host-pathogen interactions will be presented by practical demonstrations and hands-on experiments

Details
· Cell culture of human oral epithelial cell monolayers
· Preparation of fungal cells for infection
· Differential staining and fluorescence microscopy of fungal cells adhering to and invading human oral cells
· Biochemical techniques for determining the damage caused to human cells by C. albicans

· The number of participants is limited to 6 students