Practical Course Prof. Dr. Hanspeter Saluz

Cell- and Molecular Biology
Practical course on rapid heatblock thermocycling of small samples in miniaturized ultrathin-walled microplates.
In this practical course we will use a novel rapid cycling technology based on ultrathin-walled multiwell plates combined with a rapid Peltier-driven heat-block thermal cycler. The technique combines the advantages of rapid cycling, like considerably increased quality of reactions, with the advantages of conventional cyclers, e.g. simple sample handling. We will perform some single-primer and two-primer DNA fingerprinting techniques as well as sequencing reactions in an attempt to reduce reaction times as much as possible.
 

23 January 2007, HKI, LH3